Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) antibody
The Goat Polyclonal anti- antibody has been validated for ELISA, FACS, IF and WB. It is suitable to detect in samples from Human.
Catalog No. ABIN185294
Quick Overview for Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) antibody (ABIN185294)
Target
See all Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) products
Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
Reactivity
Human
Host
Goat
Clonality
Polyclonal
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
Grade
Verified
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Binding Specificity
- AA 429-442, AA 443-456
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Purpose
- LXR alpha / LXR beta
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Sequence
- CRLQDKKLPP LLSEI
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Specificity
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
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Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
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Isotype
- IgG
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Application Notes
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Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
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Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
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Alternative Name
- NR1H3, NR1H2
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Background
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
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Gene ID
- 10062, 7376
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NCBI Accession
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
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Pathways
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Target
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