Human Tissue Factor/F3 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all Tissue factor (F3) products
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
- Binding Specificity
- AA 33-251
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Tissue factor/F3 in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: NSO
Immunogen sequence: S33-E251 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Characteristics
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Sequence similarities: Belongs to the tissue factor family.
Tissue Specificity: Lung, placenta and pancreas. .
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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F3 (Human) IP-WB Antibody Pair
IP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
F3 (Human) IP-WB Antibody PairIP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
- Alternative Name
- F3 (F3 Products)
- Synonyms
- CD142 Matched Antibody Pair, TF Matched Antibody Pair, TFA Matched Antibody Pair, PRO1557 Matched Antibody Pair, PRO2086 Matched Antibody Pair, TFQTL1 Matched Antibody Pair, f3 Matched Antibody Pair, AA409063 Matched Antibody Pair, Cf-3 Matched Antibody Pair, Cf3 Matched Antibody Pair, tf Matched Antibody Pair, zgc:112151 Matched Antibody Pair, coagulation factor III, tissue factor Matched Antibody Pair, transferrin Matched Antibody Pair, coagulation factor IIIa Matched Antibody Pair, coagulation factor III Matched Antibody Pair, tissue factor Matched Antibody Pair, coagulation factor IIIb Matched Antibody Pair, coagulation factor III (thromboplastin, tissue factor) S homeolog Matched Antibody Pair, F3 Matched Antibody Pair, TF Matched Antibody Pair, f3a Matched Antibody Pair, tf Matched Antibody Pair, f3b Matched Antibody Pair, f3.S Matched Antibody Pair
- Background
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Protein Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. .
Background: Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Synonyms: Tissue factor,TF,Coagulation factor III,Thromboplastin,CD142,F3,
Full Gene Name: Tissue factor
Cellular Localisation: Isoform 1: Membrane, Single-pass type I membrane protein. - UniProt
- P13726
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