Quick Overview for DYKDDDDK Tag Peptide (ABIN1607596)
Target
Source
Application
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Peptide Type
- Synthetic
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Supplier Product No.
- 000-000-h47
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Supplier
- Rockland
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Purpose
- Triple FLAG® Peptide
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Characteristics
- synthetic FLAG® triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide
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Purification
- Greater than 90% specific peptide by HPLC and MS.
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Purity
- Greater than 90% specific peptide by HPLC and MS.
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DYKDDDDK Tag peptide
Host: Synthetic IP, SDS, WB Without preservative
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Application Notes
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Application Note: The Triple FLAG® Peptide has been tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 μg per 1.0 μL of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for recommendations for use.
Western Blot Dilution: YES as blocking peptide in PCA
Immunoprecipitation Dilution: YES - see PROTOCOL
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
-
Buffer: None
Stabilizer: None
Preservative: None -
Preservative
- Without preservative
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Storage
- -20 °C
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Storage Comment
- Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
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Expiry Date
- 6 months
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- DYKDDDDK Tag
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Target Type
- Tag
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Background
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Synonyms: synthetic FLAG® triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide
Background: The Triple FLAG® Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG® sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK). The triple FLAG peptide is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free triple FLAG® Peptide.
Target
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