Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) Peptide
GAPDH
Reactivity: Human, Mouse, Dog
Host: Synthetic
BP, Imm
Catalog No. ABIN369199
Quick Overview for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) Peptide (ABIN369199)
Target
GAPDH
(Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Origin
Human, Mouse, Dog
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- C-Term
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Sequence
- HQVVSSDFNS DT
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Characteristics
- The peptide was used in the production of ABIN369199 - Goat Anti-GAPDH (C Terminus) Loading Control Antibody. It is currently untested in blocking applications.
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Application Notes
- When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Target
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