Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) Peptide
GAPDH
Reactivity: Human, Mouse
Host: Synthetic
BP, WB
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- Target See all GAPDH products
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Protein Region
- C-Term
- Origin
- Human, Mouse
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Sequence
- FNSNSHSSTF DAGAGIALND NFVKLISWYD NEYGYSNRVV DLMAYMASKE
- Characteristics
- This is a synthetic peptide designed for use in combination with anti-Gapdh Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
- Purification
- Purified
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Concentration
- 1 mg/mL
- Buffer
- Final peptide concentration is 1 mg/mL in PBS.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Synonyms
- G3PD Peptide, GAPD Peptide, Gapd Peptide, BEST:GH12586 Peptide, CG12055 Peptide, Dmel\\CG12055 Peptide, GA3PDH Peptide, GADPH Peptide, GAP Peptide, GAPDH Peptide, GAPDH I Peptide, GAPDH-1 Peptide, GAPDH1 Peptide, GAPDHI Peptide, Gapdh Peptide, Gapdh-1 Peptide, Gapdh43E Peptide, gadph Peptide, gapdh Peptide, gapdh-1 Peptide, gh12586 Peptide, cb609 Peptide, gapd Peptide, mg:bb02e05 Peptide, wu:fb33a10 Peptide, wu:ft80f05 Peptide, KNC-NDS6 Peptide, g3pd Peptide, G3PDH Peptide, glyceraldehyde-3-phosphate dehydrogenase Peptide, Glyceraldehyde 3 phosphate dehydrogenase 1 Peptide, glyceraldehyde-3-phosphate dehydrogenase S homeolog Peptide, glyceraldehyde-3-phosphate dehydrogenase, type I Peptide, olfactory receptor 8K3 Peptide, GAPDH Peptide, Gapdh Peptide, Gapdh1 Peptide, gapdh Peptide, gapdh.S Peptide, gapDH Peptide, LOC100404960 Peptide, LOC614985 Peptide
- Background
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Gapdh has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Gapdh also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Alias Symbols: Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239
Protein Size: 333 - Molecular Weight
- 36 kDa
- Gene ID
- 14433
- NCBI Accession
- NM_008084, NP_032110
- UniProt
- P16858
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