Neurogenin 1 (NEUROG1) Peptide
NEUROG1
Reactivity: Zebrafish (Danio rerio)
Host: Synthetic
BP, Imm
Catalog No. ABIN369876
Quick Overview for Neurogenin 1 (NEUROG1) Peptide (ABIN369876)
Target
Neurogenin 1 (NEUROG1)
Origin
Zebrafish (Danio rerio)
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Sequence
- SHTDDEDSRS SLH
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Characteristics
- This peptide is the immunizing peptide for ABIN335151 and may be used for blocking experiments
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Purification
- The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Application Notes
- When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Neurogenin 1 (NEUROG1)
Target
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