Bone Morphogenetic Protein 4 (BMP4) (Internal Region) Peptide
BMP4
Reactivity: Human
Host: Synthetic
BP, Imm
Catalog No. ABIN5539389
Quick Overview for Bone Morphogenetic Protein 4 (BMP4) (Internal Region) Peptide (ABIN5539389)
Target
BMP4
(Bone Morphogenetic Protein 4 (BMP4))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- Internal Region
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Sequence
- SPKHHSQRAR K
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Predicted Reactivity
- Human
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Bone Morphogenetic Protein 4 (BMP4) (AA 293-408) peptide
BMP4 Reactivity: Human Host: Escherichia coli (E. coli) SDS
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Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 40-45 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 46.6 kDa according to NP_001193.2). Recommended concentration: 0.3-1 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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Gene ID
- 652
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NCBI Accession
- NP_001193
Target
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