NLR Family, Pyrin Domain Containing 3 (NLRP3) (Internal Region) Peptide
NLRP3
Reactivity: Human, Dog, Mouse
Host: Synthetic
BP, Imm
Catalog No. ABIN5539391
Quick Overview for NLR Family, Pyrin Domain Containing 3 (NLRP3) (Internal Region) Peptide (ABIN5539391)
Target
NLRP3
(NLR Family, Pyrin Domain Containing 3 (NLRP3))
Origin
Human, Dog, Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- Internal Region
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Sequence
- NQERTSYLEK KLSCK
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Predicted Reactivity
- Human, Mouse, Dog, Cow
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Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Preliminary experiments in Human Bone Marrow and Peripheral Blood Lymphocytes lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been re
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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Gene ID
- 114548
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NCBI Accession
- NP_004886, NP_899632, NP_001120933, NP_001120934
Target
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