NLR Family, Pyrin Domain Containing 3 (NLRP3) (Internal Region) Peptide
NLRP3
Reactivity: Human, Dog, Mouse
Host: Synthetic
BP, Imm
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- Target See all NLRP3 products
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- Protein Region
- Internal Region
- Origin
- Human, Dog, Mouse
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- NQERTSYLEK KLSCK
- Predicted Reactivity
- Human, Mouse, Dog, Cow
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- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Preliminary experiments in Human Bone Marrow and Peripheral Blood Lymphocytes lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been re
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Target
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- Gene ID
- 114548
- NCBI Accession
- NP_004886, NP_899632, NP_001120933, NP_001120934
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