Milk Fat Globule-EGF Factor 8 Protein (MFGE8) (Internal Region) Peptide
MFGE8
Reactivity: Human
Host: Synthetic
BP, Imm
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- Target See all MFGE8 products
- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
- Protein Region
- Internal Region
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- KNNSIPDKQI TAS
- Predicted Reactivity
- Human
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- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 40 kDa band observed in lysates of cell line MCF7 (calculated MW of 43.1 kDa according to NP_005919.2, and 37.5 according to NP_001108086.1 ). Recommended concentration: 1-3 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Target
- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
- Gene ID
- 4240
- NCBI Accession
- NP_005919, NP_001108086
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