Thyroid Peroxidase (TPO) (C-Term) peptide (Biotin)
TPO
Reactivity: Human
Host: Synthetic
BP, Imm
Catalog No. ABIN5539407
Quick Overview for Thyroid Peroxidase (TPO) (C-Term) peptide (Biotin) (ABIN5539407)
Target
Thyroperoxidase (TPO)
(Thyroid Peroxidase (TPO))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- C-Term
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Purification tag / Conjugate
- This Thyroperoxidase peptide is labelled with Biotin.
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Sequence
- ELRCGKHQAV GTSPQ.
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Predicted Reactivity
- Human
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Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 110 kDa band observed in Human Thyroid Gland lysates (calculated MW of 103 kDa according to NP_000538.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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