Zinc finger protein ubi-d4 (DPF2) (AA 89-103), (Internal Region) Peptide
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- Target See all DPF2 products
- DPF2 (Zinc finger protein ubi-d4 (DPF2))
- Protein Region
- AA 89-103, Internal Region
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- EDPRLSFPSI KPDTD
- Predicted Reactivity
- Human, Mouse, Rat, Dog, Pig, Cow
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- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 50 kDa and 48 kDa bands observed in lysates of cell lines Jurkat and K562 respectively (calculated MW of 44.2 kDa according to NP_006259.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Target
- DPF2 (Zinc finger protein ubi-d4 (DPF2))
- Gene ID
- 5977, 19708, 361711
- NCBI Accession
- NP_006259
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