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C-Src tyrosine Kinase (CSK) (C-Term) peptide (Biotin)

CSK Reactivity: Human, Mouse, Rat Host: Synthetic BP, Imm
Catalog No. ABIN5539443

Quick Overview for C-Src tyrosine Kinase (CSK) (C-Term) peptide (Biotin) (ABIN5539443)

Target

CSK (C-Src tyrosine Kinase (CSK))

Origin

Human, Mouse, Rat

Source

  • 6
Synthetic

Application

Blocking Peptide (BP), Immunogen (Imm)
  • Protein Region

    C-Term

    Purification tag / Conjugate

    This CSK peptide is labelled with Biotin.

    Sequence

    CEQLEHIKTH ELHL.

    Predicted Reactivity

    Human, Mouse, Rat, Dog, Cow
  • Application Notes

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 50 kDa band observed in lysates of cell line Jurkat (calculated MW of 50.7 kDa according to NP_004374.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.5-1 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    Buffer

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    Storage

    -20 °C

    Storage Comment

    Shipped at ambient temperature, store at -20°C.
  • Target

    CSK (C-Src tyrosine Kinase (CSK))

    Gene ID

    1445, 12988, 315707

    NCBI Accession

    NP_004374
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