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Periostin (POSTN) (AA 112-125), (Internal Region) Peptide

POSTN Reactivity: Human Host: Synthetic BP, Imm
Catalog No. ABIN5539455

Quick Overview for Periostin (POSTN) (AA 112-125), (Internal Region) Peptide (ABIN5539455)

Target

Periostin (POSTN)

Origin

Human

Source

  • 5
Synthetic

Application

Blocking Peptide (BP), Immunogen (Imm)
  • Protein Region

    AA 112-125, Internal Region

    Sequence

    TTQRYSDASK LREE

    Predicted Reactivity

    Human
  • Application Notes

    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 85 kDa band observed in Human Breast cancer lysates (calculated MW of 93.3 kDa according to NP_006466.2). Recommended concentration: 0.1-0.3 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.

    Buffer

    100µg of Lyophilised peptide, formulation De-ionized (DI) water.

    Storage

    -20 °C

    Storage Comment

    Shipped at ambient temperature, store at -20°C.
  • Target

    Periostin (POSTN)

    Gene ID

    10631

    NCBI Accession

    NP_006466, NP_001129406, NP_001129407, NP_001129408
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