NLR Family, Pyrin Domain Containing 3 (NLRP3) (Intermediate Domain) Peptide
NLRP3
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5684922
Quick Overview for NLR Family, Pyrin Domain Containing 3 (NLRP3) (Intermediate Domain) Peptide (ABIN5684922)
Target
NLRP3
(NLR Family, Pyrin Domain Containing 3 (NLRP3))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- Intermediate Domain
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Characteristics
- Cryopyrin peptide corresponds to 12 amino acids near the intermediate domain of Cryopyrin.
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Application Notes
- Cryopyrin Peptide is used for blocking the activity of Cryopyrin antibody . The Cryopyrin antibody ships at 0.5 mg/mL and the Cryopyrin Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Cryopyrin peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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