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Peptidylglycine alpha-Amidating Monooxygenase (PAM) Peptide

PAM Reactivity: Human Host: Synthetic BP, BI
Catalog No. ABIN5685385
  • Target See all PAM products
    PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
    Origin
    Human
    Source
    • 4
    Synthetic
    Application
    Blocking Peptide (BP), BioImaging (BI)
    Characteristics
    PAM peptide corresponds to 13 amino acids of PAM.
  • Application Notes
    PAM Peptide is used for blocking the activity of PAM antibody . The PAM antibody ships at 0.5 mg/mL and the PAM Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    PAM peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. When reconstituted minimize freezing and thawing.
  • Target
    PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
    Gene ID
    5066
    NCBI Accession
    NP_000910, NP_620121, NP_620176, NP_620177
    OMIM
    21070984
    UniProt
    P19021
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