Tripartite Motif Containing 5 (TRIM5) (Intermediate Domain) Peptide
TRIM5
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5685889
Quick Overview for Tripartite Motif Containing 5 (TRIM5) (Intermediate Domain) Peptide (ABIN5685889)
Target
TRIM5
(Tripartite Motif Containing 5 (TRIM5))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- Intermediate Domain
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Characteristics
- TRIM5 peptide corresponds to 12 amino acids near the intermediate domain of TRIM5.
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Application Notes
- TRIM5 Peptide is used for blocking the activity of TRIM5 antibody . The TRIM5 antibody ships at 0.5 mg/mL and the TRIM5 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- TRIM5 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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