Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) Peptide
UBE2I
Reactivity: Human, Mouse, Rat
Host: Synthetic
BP, BI
Catalog No. ABIN5686404
Quick Overview for Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) Peptide (ABIN5686404)
Target
UBE2I
(Ubiquitin-Conjugating Enzyme E2I (UBE2I))
Origin
Human, Mouse, Rat
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- N-Term
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Characteristics
- UBE2I peptide corresponds to 13 amino acids near the N-terminus region of UBE2I.
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Application Notes
- UBE2I Peptide is used for blocking the activity of UBE2I antibody . The UBE2I antibody ships at 0.5 mg/mL and the UBE2I Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- UBE2I peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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