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Engrailed Homeobox 2 (EN2) Peptide

EN2 Reactivity: Human, Rat Host: Synthetic BP, BI
Catalog No. ABIN5686528

Quick Overview for Engrailed Homeobox 2 (EN2) Peptide (ABIN5686528)

Target

EN2 (Engrailed Homeobox 2 (EN2))

Origin

Human, Rat

Source

  • 3
Synthetic

Application

Blocking Peptide (BP), BioImaging (BI)
  • Characteristics

    Engrailed-2 peptide corresponds to 14 amino acids of Engrailed-2.
  • Application Notes

    Engrailed-2 Peptide is used for blocking the activity of Engrailed-2 antibody . The Engrailed-2 antibody ships at 0.5 mg/mL and the Engrailed-2 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Engrailed-2 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. When reconstituted minimize freezing and thawing.
  • Target

    EN2 (Engrailed Homeobox 2 (EN2))

    Gene ID

    2020

    NCBI Accession

    NP_001418

    OMIM

    7710121

    UniProt

    P19622
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