14-3-3 theta (YWHAQ) Peptide
YWHAQ
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5686555
Quick Overview for 14-3-3 theta (YWHAQ) Peptide (ABIN5686555)
Target
14-3-3 theta (YWHAQ)
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Characteristics
- 14-3-3 tau peptide corresponds to 11 amino acids of 14-3-3 tau.
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Application Notes
- 14-3-3 tau Peptide is used for blocking the activity of 14-3-3 tau antibody . The 14-3-3 tau antibody ships at 0.5 mg/mL and the 14-3-3 tau Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 14-3-3 tau peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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