Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) Peptide
ITCH
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5686623
Quick Overview for Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) Peptide (ABIN5686623)
Target
ITCH
(Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Characteristics
- AIF4 peptide corresponds to 13 amino acids of AIF4.
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Application Notes
- AIF4 Peptide is used for blocking the activity of AIF4 antibody . The AIF4 antibody ships at 0.5 mg/mL and the AIF4 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- AIF4 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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