Gap Junction Protein, alpha 1, 43kDa (GJA1) Peptide
GJA1
Reactivity: Human, Rat
Host: Synthetic
BP, BI
Catalog No. ABIN5686796
Quick Overview for Gap Junction Protein, alpha 1, 43kDa (GJA1) Peptide (ABIN5686796)
Target
Connexin 43/GJA1 (GJA1)
(Gap Junction Protein, alpha 1, 43kDa (GJA1))
Origin
Human, Rat
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Characteristics
- GJA1 peptide corresponds to 14 amino acids of GJA1.
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Application Notes
- GJA1 Peptide is used for blocking the activity of GJA1 antibody . The GJA1 antibody ships at 0.5 mg/mL and the GJA1 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- GJA1 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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