Hepatocyte Nuclear Factor 4, alpha (HNF4A) Peptide
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- Target See all HNF4A products
- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), BioImaging (BI)
- Characteristics
- HNF4A peptide corresponds to 16 amino acids of HNF4A.
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- Application Notes
- HNF4A Peptide is used for blocking the activity of HNF4A antibody . The HNF4A antibody ships at 0.5 mg/mL and the HNF4A Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- HNF4A peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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