Interleukin-27 subunit beta (IL-27b) Peptide
IL-27b
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5686891
Quick Overview for Interleukin-27 subunit beta (IL-27b) Peptide (ABIN5686891)
Target
EBI3 (IL-27b)
(Interleukin-27 subunit beta (IL-27b))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Characteristics
- EBI3 peptide corresponds to 15 amino acids near an internal region of EBI3.
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Application Notes
- EBI3 Peptide is used for blocking the activity of EBI3 antibody . The EBI3 antibody ships at 0.5 mg/mL and the EBI3 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- EBI3 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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