SMAD Family Member 4 (SMAD4) (C-Term) Peptide
SMAD4
Reactivity: Human, Mouse
Host: Synthetic
BP, BI
Catalog No. ABIN5686974
Quick Overview for SMAD Family Member 4 (SMAD4) (C-Term) Peptide (ABIN5686974)
Target
SMAD4
(SMAD Family Member 4 (SMAD4))
Origin
Human, Mouse
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- C-Term
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Characteristics
- SMAD4 peptide corresponds to 12 amino acids near the C-terminus region of SMAD4.
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Application Notes
- SMAD4 Peptide is used for blocking the activity of SMAD4 antibody . The SMAD4 antibody ships at 0.5 mg/mL and the SMAD4 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- SMAD4 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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