Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) Peptide
Quick Overview for Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) Peptide (ABIN5687017)
Target
Origin
Source
Application
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Protein Region
- C-Term
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Characteristics
- Nucleophosmin peptide corresponds to 14 amino acids near the C-terminus region of Nucleophosmin.
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Application Notes
- Nucleophosmin Peptide is used for blocking the activity of Nucleophosmin antibody . The Nucleophosmin antibody ships at 0.5 mg/mL and the Nucleophosmin Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Nucleophosmin peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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