phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Intermediate Domain) Peptide
PCK1
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5687046
Quick Overview for phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Intermediate Domain) Peptide (ABIN5687046)
Target
PCK1
(phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- Intermediate Domain
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Characteristics
- PCK1 peptide corresponds to 14 amino acids near the intermediate domain of PCK1.
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Application Notes
- PCK1 Peptide is used for blocking the activity of PCK1 antibody . The PCK1 antibody ships at 0.5 mg/mL and the PCK1 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- PCK1 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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