Gastrin-Releasing Peptide (GRP) Peptide
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- Target See all Gastrin-Releasing Peptide (GRP) products
- Gastrin-Releasing Peptide (GRP)
- Peptide Type
- Synthetic
- Origin
- Bombina bombina (Fire-bellied toad)
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Source
- Synthetic
- Application
- Blocking Peptide (BP)
- Purpose
- A Ligand of the Bombesin G-Protein Coupled Receptor
- Sequence
- EQRLGNQWAV GHLM-OH
- Specificity
- Toxin target: Bombesin receptors, Glu1 - Pyrrolidone carboxylic acid
- Characteristics
- A Ligand of the Bombesin G-Protein Coupled Receptor
- Purity
- ≥98 % (HPLC)
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Gastrin-Releasing Peptide (GRP) peptide (Ovalbumin)
GRP Reactivity: Human Host: Synthetic WB, Imm, SDS
Gastrin-Releasing Peptide (GRP) peptide (Ovalbumin)GRP Reactivity: Rat Host: Synthetic WB, Imm, SDS
Gastrin-Releasing Peptide (GRP) peptide (Ovalbumin)GRP Reactivity: Mouse Host: Synthetic WB, Imm, SDS
Gastrin-Releasing Peptide (GRP) peptide (BSA)GRP Reactivity: Human Host: Synthetic WB, Imm, SDS
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- Application Notes
- Antigen Preadsorption Control: 1 - 100 nM
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
- Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
- Storage
- 4 °C,-20 °C
- Storage Comment
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Target
- Gastrin-Releasing Peptide (GRP)
- Background
- Bombesin is an active neuropeptide originally isolated from amphibian skin but also found in the central and peripheral nervous systems of amphibians1. Later it was established that mammals and birds express two family groups of endogenous Bombesin-like factors, the gastrin-releasing peptides and neuromedin B2. The biological activities of bombesin are mediated through a family of three specific G-protein coupled receptors (GPCRs): BB1 which is also known as a neuromedin B receptor, BB2 also known as a gastrin-releasing peptide receptor and BB3 which is a low affinity binder for the Bombesin-like factors3,4.Bombesin and Bombesin-like factors show a broad range of biological activities such as regulation of smooth muscle contraction, stimulation of neuropeptide and hormone secretion, modulation of neural activity, growth regulation, and mediate anorexic effects in the hypothalamus5.
- Molecular Weight
- 1620.8 Da
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