Apamin peptide (Atto 488)
Reactivity: Bee
Host: Synthetic
BP, FACS, IF, FM, LCI
Catalog No. ABIN7582128
Quick Overview for Apamin peptide (Atto 488) (ABIN7582128)
Target
Apamin
Origin
Bee
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF), Fluorescence Microscopy (FM), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 488
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Purpose
- A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to the Fluorescent Dye ATTO-488
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Sequence
- CNCKAPETAL CARRCQQH-NH2
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Specificity
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Toxin target: KCa2 K+ channels,
Disulfide bonds between: Cys1-Cys11, and Cys3-Cys15 His18 - C-terminal amidation ATTO Fluor-488 -
Characteristics
- A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to the Fluorescent Dye
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Apamin peptide
Reactivity: Bee Host: Synthetic BP
Apamin peptide (Biotin)Reactivity: Bee Host: Synthetic FACS, IF
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Application Notes
- Antigen Preadsorption Control: 1 - 50 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- Apamin
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Background
- APM, Apamine,Apamin is a natural peptide isolated and purified from Apis mellifera bee venoM Apamin blocks small conductance Ca2+-activated K+ channels (SK). It is specific for the SK1-3 isoforms, but ineffective in blocking other calcium activated K+ channels2. In mammalian cell lines, Apamin blocks expressed hSK1 with an IC50 of ~10 nM3, rSK1 and rSK2 with IC50 of ~3 and
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Molecular Weight
- 2601 Da
Target
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