Catalog No. ABIN7582129
Quick Overview for Apamin peptide (Biotin) (ABIN7582129)
Target
Apamin
Origin
Bee
Source
Synthetic
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Biotin
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Purpose
- A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin
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Sequence
- Biotin-CNCKAPETAL CARRCQQH-NH2
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Specificity
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Toxin target: KCa2 K+ channels,
Disulfide bonds between: Cys1-Cys11, and Cys3-Cys15 His18 - C-terminal amidation LC-Biotin -
Characteristics
- A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin
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Apamin peptide (Atto 488)
Reactivity: Bee Host: Synthetic BP, FACS, IF, FM, LCI
Apamin peptideReactivity: Bee Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 1 - 50 nM
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Comment
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Electrophysiology
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution for the shortest time possible at -20°C. Avoid multiple freeze- thaw cycles. We do not recommend storing the toxin in working solutions for longer than a day.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
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- Apamin
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Background
- APM, Apamine,Apamin is a natural peptide isolated and purified from Apis mellifera bee venoM Apamin blocks small conductance Ca2+-activated K+ channels (SK). It is specific for the SK1-3 isoforms, but ineffective in blocking other calcium activated K+ channels2. In mammalian cell lines, Apamin blocks expressed hSK1 with an IC50 of ~10 nM3, rSK1 and rSK2 with IC50 of ~3 and
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Molecular Weight
- 2367 Da
Target
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