alpha-conotoxin GI peptide (Atto 647N)
Reactivity: Conus geographus (Geography cone)
Host: Synthetic
BP, FACS, FM, IF, LCI
Catalog No. ABIN7582173
Quick Overview for alpha-conotoxin GI peptide (Atto 647N) (ABIN7582173)
Target
alpha-conotoxin GI
Origin
Conus geographus (Geography cone)
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 647N
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Sequence
- ECCNPACGRH YSC-NH2
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Specificity
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Toxin target: α1/β1/δ/γ nAChR, Cys13 - C-terminal amidation ATTO-647N
Disulfide bonds between: Cys2-Cys7, Cys3-Cys13
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alpha-conotoxin GI peptide (FITC)
Reactivity: Conus geographus (Geography cone) Host: Synthetic BP, FACS, FM, IF
alpha-conotoxin GI peptideReactivity: Conus geographus (Geography cone) Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 400 nM- 4 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- alpha-conotoxin GI
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Background
- G1, Alpha-conotoxin GI,α-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks α/δ nicotinic ACh channel receptors (nAChR). α-Conotoxin GI reversibly inhibits the high affinity α/δ site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (α/γ site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by α-Conotoxin GI5.
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Molecular Weight
- 2065 Da
Target
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