Catalog No. ABIN7582204
Quick Overview for GsMTx-4 Peptide (ABIN7582204)
Target
GsMTx-4
Origin
Grammostola rosea
Source
Synthetic
Application
Blocking Peptide (BP)
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Peptide Type
- Synthetic
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Purpose
- A Blocker of NaV1.7, TRPC1, TRPC6 and Piezo1 Channels
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Sequence
- GCLEFWWKCN PNDDKCCRPK LKCSKLFKLC NFSF-NH2
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Specificity
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Toxin target: NaV1.7 channels, TRPC1, TRPC6, Piezo1,
Disulfide bonds between: Cys2-Cys17, Cys9-Cys23, and Cys16-Cys30 Phe34 - C-terminal amidation -
Characteristics
- A Blocker of NaV1.7, TRPC1, TRPC6 and Piezo1 Channels
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Purity
- ≥98 % (HPLC)
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GsMTx-4 peptide (Atto 647N)
Reactivity: Grammostola rosea Host: Synthetic BP, FACS, FM, IF, LCI
GsMTx-4 peptide (Biotin)Reactivity: Grammostola rosea Host: Synthetic BP, FACS, FM, IF, LCI
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Application Notes
- Antigen Preadsorption Control: 500 nM - 10 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- 4 °C,-20 °C
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Storage Comment
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- GsMTx-4
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Background
- M-TRTx-Gr1a, M-theraphotoxin-Gr1a, GsMTx4,GsMTx-4 is a 34 amino acid peptidyl toxin originally isolated from the Grammostola rosea (Chilean rose) tarantula venom and belongs to the huwentoxin-1 family1.This toxin inhibits different channels and in addition has antimicrobial activity. It blocks cation-selective mechanosensitive ion channels (strech-activated channels, SACs), without having an effect on whole-cell voltage-sensitive currents1. In addition, it inhibits atrial fibrillation2 as well as the membrane motor of outer hair cells3 at low doses. A medium toxicity on a large spectra of voltage-gated Na+ channels, namely NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6 and NaV1.7 was reported. GsMTx-4 also inhibits K+ channels KV11.1 and KV11.2, whereas it does not inhibit K+ channels KV1.1 (IC50 > 85 μM), KV1.4 (IC50 > 85 μM) and KV11.3 (IC50 = 53 μM)4. GsMTx-4 was also found to inhibit both TRPC1 and TRPC6 channels5,6, as well as Piezo1, the mechanosensitive channel7.Antimicrobial activity is shown against several Gram-positive and Gram-negative bacteria as well8.
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Molecular Weight
- 4096 Da
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UniProt
- Q7YT39
Target
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