MmTx1 Toxin peptide (Atto 488)
Reactivity: Micrurus mipartitus
Host: Synthetic
BP, FACS, IF, LCI
Catalog No. ABIN7582257
Quick Overview for MmTx1 Toxin peptide (Atto 488) (ABIN7582257)
Target
MmTx1 Toxin
Origin
Micrurus mipartitus
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 488
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Purpose
- A Super Potent Allosteric Modulator of GABA(A) Receptors Conjugated to the Fluorescent Dye ATTO-488
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Sequence
- LTCKTCPFTT CPNSESCPGG QSICYQRKWE EHRGERIERR CVANCPAFGS HDTSLLCCTR DNCN-OH
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Specificity
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Toxin target: GABA(A) receptors,
Disulfide bonds between: Cys3-Cys24, Cys6-Cys11, Cys17-Cys41, Cys45-Cys57, Cys58-Cys63 ATTO Fluor-488 -
Characteristics
- A Super Potent Allosteric Modulator of GABA(A) Receptors Conjugated to the Fluorescent Dye
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MmTx1 Toxin peptide
Reactivity: Micrurus mipartitus Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 200 - 400 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- MmTx1 Toxin
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Background
- Micrurotoxin 1,MmTx1 (Micrurotoxin 1) is a peptide toxin originally isolated from Micrurus mipartitus (Red-tailed coral snake) venoM The toxin is an allosteric modulator of γ-aminobutyric acid type A receptors (GABA(A)) and tightly binds to GABA(A) receptors at subnanomolar concentrations. MmTx1 allosterically increases GABA(A) receptor susceptibility to agonistic actions, thereby potentiating receptor opening and desensitization by interacting with the α+/β-interface, a benzodiazepine-like binding site1. MmTx1 may be a priceless tool in evoking seizures for testing novel antiepileptic drugs or as lead molecules for designing therapeutics that modulate GABA(A) receptor activity1.GABA(A) receptors belong to the cys-loop pentameric ligand-gated ion channel family. These receptors are major inhibitory neurotransmitter receptors in the brain and in the mammalian central nervous system and are responsible for the mediation of GABA action, a major inhibitory neurotransmitter, through the central nervous system2.
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Molecular Weight
- ~7775 Da
Target
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