Stichodactyla Toxin peptide (Biotin)
Reactivity: Stichodactyla helianthus
Host: Synthetic
BP, FACS, IF
2 images
Catalog No. ABIN7582283
Quick Overview for Stichodactyla Toxin peptide (Biotin) (ABIN7582283)
Target
Stichodactyla Toxin
Origin
Stichodactyla helianthus
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunofluorescence (IF)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Biotin
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Purpose
- Biotinylated ShK Conjugate for Sensitive Detection of KV Channels
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Sequence
- RSCIDTIPKS RCTAFQCKHS MKYRLSFCRK TCGTC-OH
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Specificity
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Toxin target: KV1.1, KV1.3, KV1.4, KV1.6 channels,
Disulfide bonds between Cys3-Cys35, Cys12-Cys28, and Cys17-Cys32 LC-Biotin -
Characteristics
- Biotinylated ShK Conjugate for Sensitive Detection of KV Channels
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Stichodactyla Toxin peptide (Atto 590)
Reactivity: Stichodactyla helianthus Host: Synthetic BP, FACS, IF, LCI
Stichodactyla Toxin peptideReactivity: Stichodactyla helianthus Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 1 - 100 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.,Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
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- Stichodactyla Toxin
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Background
- K+ channel toxin ShK, Toxin κ-stichotoxin-She3a, Kappa-stichotoxin-She3a, κ-SHTX-She3a,ShK (Stichodactyla Toxin) is a peptidyl toxin originally isolated from the nematocyst of the sea anemone Stichodactyla helianthus.1ShK blocks KV1.3, KV1.1, KV1.4, and KV1.6 at subnanomolar concentrations and KV3.2 channels at 1000-fold higher concentration than that required to inhibit KV1.3 channels. It has been shown to block KV current in DRG neurons, to displace radioactive Dendrotoxin from brain synaptosomes1 and inhibit I125-Charybdotoxin binding to Jurkat T lymphocytes with an IC50 of 32 pM2,4Evidence also suggests that native KV currents in the central nervous system, which are predominantly carried by KV1.2 channels, are highly sensitive to this toxin.5A fluorescently labeled synthetic ShK is used to recognize and to sort KV1.3 containing lymphocytes.4,6
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Molecular Weight
- 4563 Da
Target
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