Tertiapin Q peptide (Atto 633)
Reactivity: Bee
Host: Synthetic
BP, FACS, FM, IF, LCI
Catalog No. ABIN7582294
Quick Overview for Tertiapin Q peptide (Atto 633) (ABIN7582294)
Target
Tertiapin Q
Origin
Bee
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 633
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Purpose
- An Inward Rectifier K+ Channel Blocker Conjugated to the Fluorescent Dye ATTO-633
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Sequence
- ALCNCNRIII PHQCWKKCGK K-NH2
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Specificity
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Toxin target: Kir1.1, Kir3.2 K+ channels,
Disulfide bonds between: Cys3-Cys14, Cys5-Cys18 Lys21 - C-terminal amidation ATTO Fluor-633 -
Characteristics
- An Inward Rectifier K+ Channel Blocker Conjugated to the Fluorescent Dye
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Tertiapin Q peptide (Atto 488)
Reactivity: Bee Host: Synthetic BP, FACS, FM, IF, LCI
Tertiapin Q peptideReactivity: Bee Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 50 - 200 nM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- Tertiapin Q
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Background
- Tertiapin, the native toxin, was originally isolated from European honey bee Apis mellifera venoM Native and synthetic Tertiapin blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 (Kir1.1, IC50 = 2 nM) and GIRK (Kir3 family, IC50 for the Kir3.1/3.4 heteromer was 8.6 nM) but with no effect on the Kir2 family member1. In accordance, it was shown to inhibit acetylcholine induced K+ currents in mammalian cardiomyocytes2,3.Tertiapin-Q is a derivative of Tertiapin in which Met13 is substituted by a Gln residue. However, unlike native Tertiapin, Tertiapin-Q is non-oxidizable and therefore is more stable4.Tertiapin-Q inhibits the above-mentioned channels with similar affinities and also inhibits Ca2-activated large conductance BK-type K+ channels in a concentration and voltage-dependent manner5.
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Molecular Weight
- 3159.6 Da
Target
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