Cyclophilin B (PPIB) (AA 26-216) Peptide
PPIB
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
Catalog No. ABIN934947
Quick Overview for Cyclophilin B (PPIB) (AA 26-216) Peptide (ABIN934947)
Target
PPIB
(Cyclophilin B (PPIB))
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Biological Activity
- Active
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Protein Region
- AA 26-216
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Sequence
- MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
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Characteristics
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Purified recombinant Human Cyclophilin B protein
Expression System: E.coli
Bioactivity: Specific activity is > 170 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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Application Notes
- Cyclophilin B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Assay Procedure
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- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
2. Add recombinant Cyclophilin B protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
5. Record the increase in A410 nm for 20 minutes
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris, pH 8.0, containing 20 mM NaCl, 0.0 mM DTT, and 10 % glycerol.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- PPIB (Cyclophilin B (PPIB))
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Background
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Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Cphn 2 protein, AA408962 protein, CYP S1 protein, Cyclophilin B protein, PPIB protein, MGC14109 protein, S cyclophilin., 26- 216aa protein, PPIase protein, Cphn2 protein, CyP 20b protein, SCYLP protein, AA553318 protein, AI844835 protein, Peptidyl prolyl cis trans isomerase B precursor protein, Peptidylprolyl isomerase B protein, Rotamase protein, CYPB protein, MGC2224 protein, peptidyl prolyl cis trans isomerase B protein, CYP-S1: Cyclophilin-B protein -
Molecular Weight
- 21.2 kDa (192 AA)
Target
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