Thioredoxin 2 (TXN2) (AA 60-166) Peptide
TXN2
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
1 image
Catalog No. ABIN934974
Quick Overview for Thioredoxin 2 (TXN2) (AA 60-166) Peptide (ABIN934974)
Target
TXN2
(Thioredoxin 2 (TXN2))
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Biological Activity
- Active
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Protein Region
- AA 60-166
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Sequence
- MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
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Characteristics
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Purified recombinant Human Thioredoxin 2 protein
Expression System: E.coli
Bioactivity: Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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Application Notes
- Thioredoxin 2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Assay Procedure
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- Prepare a 1 mL reaction cocktail in a suitable container: The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
2. Add 4 µg of recombinant thioredoxin-2 into reaction cocktail and mix immediately.
3. Incubate at 25 °C for 20 minutes.
4. Record the increase in A650 nm for 15 minutes.
- Prepare a 1 mL reaction cocktail in a suitable container: The final concentrations are 1 mM Tris, 61 mM sodium phosphate, 1 mM DTT, 0.1 % (w/v) insulin, 1 mM EDTA.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in PBS, pH 7.4.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- TXN2 (Thioredoxin 2 (TXN2))
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Background
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Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin 2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin 2 was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Thioredoxin 2, MTRX protein, MT-TRX protein, Thioredoxin2 protein, TXN 2 protein, Thioredoxin mitochondrial protein, Thioredoxin-2, Thioredoxin 2 protein, thioredoxin 2 precursor protein, mitochondrial thioredoxin protein, Thioredoxin -2, TRX2 protein, TRX 2 protein, Thioredoxin -2 protein, Thioredoxin 2, TXN2 protein -
Molecular Weight
- 11 kDa (108 AA)
Target
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