Protein A (AA 37-469) Peptide
Reactivity: Staphylococcus aureus
Host: Escherichia coli (E. coli)
SDS
1 image
Catalog No. ABIN935031
Quick Overview for Protein A (AA 37-469) Peptide (ABIN935031)
Target
Protein A
Origin
Staphylococcus aureus
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Protein Region
- AA 37-469
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Sequence
- MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET
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Characteristics
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Purified recombinant S.aureus Protein A protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 90 % pure
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Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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Application Notes
- Protein A protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Protein A
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Background
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Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: SPA protein, Immunoglobulin G binding protein A protein, , Immunoglobulin G binding protein A Staphylococcal protein A protein -
Molecular Weight
- 48.1 kDa (434 AA)
Target
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