Peroxiredoxin 3 (PRDX3) (AA 63-256) Peptide
PRDX3
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
1 image
Catalog No. ABIN935037
Quick Overview for Peroxiredoxin 3 (PRDX3) (AA 63-256) Peptide (ABIN935037)
Target
Peroxiredoxin 3 (PRDX3)
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Biological Activity
- Active
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Protein Region
- AA 63-256
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Sequence
- MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ
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Characteristics
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Purified recombinant Human Peroxiredoxin 3 protein
Expression System: E.coli
Bioactivity: Specific activity: approximately 82-83 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 °C for 1 minute
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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Application Notes
- Peroxiredoxin 3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Peroxiredoxin 3 (PRDX3)
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Background
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Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. Recombinant human Peroxiredoxin 3 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: MER5 protein, AOP1 protein, SP22 protein, Peroxiredoxin -3 protein, Peroxiredoxin 3, PRDX3 protein, PRX III protein, PRX3 protein, Peroxiredoxin 3 protein, Peroxiredoxin-3, mitochondrial Antioxidant Protein 1 protein, Antioxidant protein 1 (AOP-1) protein, Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase protein, Peroxiredoxin 3 protein, Peroxiredoxin -3, Thioredoxin-dependent peroxide reductase mitochondrial protein -
Molecular Weight
- 21.5 kDa (195 AA)
Target
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