Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3) (C-Term) Peptide
UCP3
Reactivity: Mammalian
Host: Synthetic
BP, ELISA, WB, IF, IHC
Sodium azide
Catalog No. ABIN935880
Quick Overview for Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3) (C-Term) Peptide (ABIN935880)
Target
UCP3
(Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))
Origin
Mammalian
Source
Synthetic
Application
Blocking Peptide (BP), ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
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Peptide Type
- Synthetic
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Protein Region
- C-Term
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Cross-Reactivity (Details)
- This human UCP3 peptide sequences is 100 % conserved in long and short form of human UCP3, 100 % in pig, and canine, 92 % in mouse, rat, and bovine UCP3. It has no significant homology with UCP1-2 or UCP4-5. This peptide is not suitable for western blot because of its low mol. Wt.
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Characteristics
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A synthetic UCP3 C-terminal Blocking peptide for use as a blocking control in assays to test for specificity of UCP3 C-terminal antibody,
Alternative Names: UCP-3 Blocking peptide, UCP 3 Blocking peptide, Uncoupling Protein 3 Blocking peptide, UCP3 Control peptide, UCP3, UCP-3, UCP 3, UCP-3 Blocking Peptide, UCP 3 Blocking Peptide
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Application Notes
- ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
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Assay Procedure
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UCP3 C-terminal Blocking peptide, catalog no. ABIN935880 is also available for use as a blocking control in assays to test for the specificity of this UCP3 C-terminal antibody
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 100 µL of water to 1 mg/mL.
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Buffer
- Lyophilized from PBS, pH 7.5, with 0.05 % NaN3.
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
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- UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))
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Background
- Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 AA long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation.
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Molecular Weight
- <3 kDa
Target
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