Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) Peptide
UCHL1
Reactivity: Mammalian
Host: Synthetic
BP
Catalog No. ABIN936429
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20 μg
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Quick Overview for Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) Peptide (ABIN936429)
Target
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Origin
Mammalian
Source
Synthetic
Application
Blocking Peptide (BP)
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Peptide Type
- Synthetic
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Sequence
- GASSEDTLLK DAAKVCR
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Characteristics
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A synthetic peptide for use as a blocking control in assays to test for specificity of PGP 9.5 antibody,
Alternative Names: Ubiquitin carboxyl-terminal esterase L1 Blocking Peptide, Neuron cytoplasmic protein 9.5 Blocking Peptide, Ubiquitin thioesterase L1 Blocking Peptide, PARK5 Blocking Peptide, Ubiquitin C-terminal hydrolase Blocking Peptide, UCH-L1 Blocking Peptide
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Application Notes
- 2 mg/mL is recommended for most absorption control applications.
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Assay Procedure
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Antibody should be pre-incubated with peptide prior to incubation with tissue/sample. We suggest perincubation for 1 hour at room temperature. A 10^-6 or 10^-5 molar (M) peptide concentration is suggested to block antigen/antibody binding. However, optimal working concentration should be determined by the investigator using a range of peptide dilutions to eliminate or reduce immunoreactivity.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with deionized water.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20 °C.
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- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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Background
- This enzyme is a thiol protease that hydrolyzes apeptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cellsof the diffuse neuroendocrine system.
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Molecular Weight
- 1.763 kDa
Target
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