Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) Peptide
UCHL1
Reactivity: Mammalian
Host: Synthetic
BP
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- Target See all UCHL1 products
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
- Peptide Type
- Synthetic
- Origin
- Mammalian
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Source
- Synthetic
- Application
- Blocking Peptide (BP)
- Sequence
- GASSEDTLLK DAAKVCR
- Characteristics
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A synthetic peptide for use as a blocking control in assays to test for specificity of PGP 9.5 antibody,
Alternative Names: Ubiquitin carboxyl-terminal esterase L1 Blocking Peptide, Neuron cytoplasmic protein 9.5 Blocking Peptide, Ubiquitin thioesterase L1 Blocking Peptide, PARK5 Blocking Peptide, Ubiquitin C-terminal hydrolase Blocking Peptide, UCH-L1 Blocking Peptide
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- Application Notes
- 2 mg/mL is recommended for most absorption control applications.
- Assay Procedure
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Antibody should be pre-incubated with peptide prior to incubation with tissue/sample. We suggest perincubation for 1 hour at room temperature. A 10^-6 or 10^-5 molar (M) peptide concentration is suggested to block antigen/antibody binding. However, optimal working concentration should be determined by the investigator using a range of peptide dilutions to eliminate or reduce immunoreactivity.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with deionized water.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
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- Target
- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
- Background
- This enzyme is a thiol protease that hydrolyzes apeptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cellsof the diffuse neuroendocrine system.
- Molecular Weight
- 1.763 kDa
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