Non-POU Domain Containing, Octamer-Binding (NONO) Peptide
NONO
Reactivity: Mammalian
Host: Synthetic
BP, WB, IHC
Catalog No. ABIN939996
Quick Overview for Non-POU Domain Containing, Octamer-Binding (NONO) Peptide (ABIN939996)
Target
NONO
(Non-POU Domain Containing, Octamer-Binding (NONO))
Origin
Mammalian
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB), Immunohistochemistry (IHC)
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Peptide Type
- Synthetic
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Sequence
- KQNHTPRKHH QHHHQQQHHQ QQQQQPPPPP IPANGQQASS QNEGLTIDLK
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Characteristics
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A synthetic peptide for use as a blocking control in assays to test for specificity of NONO antibody,
Alternative Names: NONO control peptide, NONO antibody Blocking Peptide, Anti-NONO Blocking Peptide, Non-Pou Domain Containing Octamer-Binding Blocking Peptide -
Purification
- The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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Application Notes
- Optimal conditions should be determined by the investigator
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 µL of distilled water for a final peptide concentration is 1 mg/mL.
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Buffer
- PBS
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C long term.
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- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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Background
- NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
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Molecular Weight
- 52 kDa
Target
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