conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Peptide
Quick Overview for conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) Peptide (ABIN964095)
Target
Source
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Peptide Type
- Synthetic
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Supplier Product No.
- 100-401-219p
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Supplier
- Rockland
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Purpose
- IKKα Peptide
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Characteristics
- Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, control peptide, blocking peptide
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Purification
- Greater than 95% specific peptide
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Purity
- Greater than 95% specific peptide
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Sterility
- Sterile filtered
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Application Notes
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Application Note: Control peptide should be used at 1.0 μg per 1.0 μL of antiserum in per assay.
Other: Control peptide should be used at 1.0 μg per 1.0 μL of antiserum per assay.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 6 months
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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Background
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Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, control peptide, blocking peptide
Background: Intended for use as a control peptide when used with anti-IKKa to block specific interaction of anti-IKKalpha [p/n 100-401-219]. NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB. In turn IkB is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
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UniProt
- O15111
Target
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