Calreticulin (CALR) (Middle Region) Peptide
CALR
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN974410
Quick Overview for Calreticulin (CALR) (Middle Region) Peptide (ABIN974410)
Target
Calreticulin (CALR)
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Sequence
- PDPSIYAYDN FGVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-CALR Antibody(ARP58124_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Calreticulin (CALR) peptide (Ovalbumin)
CALR Reactivity: Human Host: Synthetic WB, Imm, SDS
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Calreticulin (CALR)
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Background
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Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Alias Symbols: CRT, FLJ26680, RO, SSA, cC1qR
Protein Interaction Partner: CALR,HLA-A,PDIA3,TSHR,APOB,APP,AR,B2M,CALR,CD1D,F5,F8,FBN1,FGB,HLA-C,ITGA2B,ITGA3,ITGAV,LPL,LRP1,MBL2,MTNR1A,NKX2-1,NR3C1,PDIA2,PDIA3,PLAT,PLP1,PRF1,SCARF1,SLC2A1,SLC6A4,SUMO4,TAPBP,TF,THBS1,TSHR,VWF,APOB,APP,AR,CANX,CD1D,CD40,ELF3,EPB41,F5,FGB,H2AFX,LRP1
Protein Size: 417 -
Molecular Weight
- 46 kDa
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Gene ID
- 811
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NCBI Accession
- NM_004343, NP_004334
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UniProt
- P27797
Target
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