DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Peptide
DFFA
Reactivity: Human
Host: Synthetic
BP, WB
Quick Overview for DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Peptide (ABIN975735)
Target
DFFA
(DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-DFFA antibody (Catalog #: ARP30359_T100). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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Background
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Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DFF-45, DFF1, Ion ChannelAD, ICAD
Protein Interaction Partner: CASP3,CASP7,CIDEB,DFFB,EWSR1,GZMB,NECAB2,STRN4,YWHAG,YWHAZ,BAK1,CIDEB,DFFB,EWSR1,HERC1,MCC,NAP1L5,NECAB2,TMBIM4,USP15,YWHAZ
Protein Size: 331 -
Molecular Weight
- 37 kDa
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Gene ID
- 1676
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NCBI Accession
- NM_004401, NP_004392
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UniProt
- O00273
Target
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