DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) Peptide
DNMT1
Reactivity: Mouse
Host: Synthetic
BP, WB
Catalog No. ABIN975937
Quick Overview for DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) Peptide (ABIN975937)
Target
DNMT1
(DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
Origin
Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Sequence
- SSVATRRTTR QTTITAHFTK GPTKRKPKEE SEEGNSAESA AEERDQDKKR
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Dnmt1 Antibody(ARP37033_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
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Background
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Dnmt1 methylates CpG residues.Dnmt1 preferentially methylates hemimethylated DNA. Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance.Dnmt1 associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones.Dnmt1 mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, Dnmt1 is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Alias Symbols: Cxxc9, Dnmt, Dnmt1o, MTase, Met-1, Met1, MommeD2, MCMT, m.MmuI
Protein Interaction Partner: HDAC1
Protein Size: 1619 -
Molecular Weight
- 183 kDa
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Gene ID
- 13433
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NCBI Accession
- NM_010066, NP_034196
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UniProt
- P13864
Target
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