DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) (Middle Region) Peptide
DNMT3B
Reactivity: Human
Host: Synthetic
BP, WB
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- Target See all DNMT3B products
- DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))
- Protein Region
- Middle Region
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Characteristics
- This is a synthetic peptide designed for use in combination with anti-DNMT3B antibody (Catalog #: ARP49124_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
- Purification
- Purified
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Concentration
- 1 mg/mL
- Buffer
- Final peptide concentration is 1 mg/mL in PBS.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))
- Background
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DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Alias Symbols: ICF, M.HsaIIIB, ICF1
Protein Interaction Partner: DNMT1,DNMT3L,SUMO1,UBE2I,CBX1,CBX5,DNMT1,DNMT3A,DNMT3L,EED,EZH2,HDAC1,HDAC2,NCAPG,SMARCA5,SUMO1,SUV39H1,UBE2I,ZNF238,CBX1,CBX3,CBX5,CBX5,DNMT1,DNMT1,DNMT3A,DNMT3L,HDAC1,HDAC1,HDAC2,KIF4A,MBD4,NCAPG,RARB,SIN3A,SMARCA5,SMARCA5,SMC2,SMC4,SPI1,SUMO1,TDG,UBE2I
Protein Size: 770 - Molecular Weight
- 86 kDa
- Gene ID
- 1789
- NCBI Accession
- NM_175849, NP_787045
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