Crossover junction endonuclease EME1 (EME1) (Middle Region) Peptide
EME1
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN976319
Quick Overview for Crossover junction endonuclease EME1 (EME1) (Middle Region) Peptide (ABIN976319)
Target
Crossover junction endonuclease EME1 (EME1)
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-EME1 antibody (Catalog #: ARP52988_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Crossover junction endonuclease EME1 (EME1)
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Background
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EME1 interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM].
Alias Symbols: FLJ31364, MMS4L, SLX2A
Protein Interaction Partner: MUS81,BTBD12,GIYD2,MUS81
Protein Size: 570 -
Molecular Weight
- 63 kDa
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Gene ID
- 146956
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NCBI Accession
- NM_152463, NP_689676
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UniProt
- Q96AY2
Target
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