Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) Peptide
PDHA1
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN981527
Quick Overview for Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1) Peptide (ABIN981527)
Target
PDHA1
(Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-PDHA1 antibody (Catalog #: ARP48137_T100). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))
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Background
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The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1), dihydrolipoyl transacetylase (DLAT, MIM 608770) (E2, EC 2.3.1.12), and dihydrolipoyl dehydrogenase (DLD, MIM 238331) (E3, EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PDHA, PDHCE1A, PHE1A
Protein Interaction Partner: PDK3,DLAT,EIF6,PDHA1,PDHB,PDK1,PDK2,PDK3,PDK4,PDP1,C19orf62,DGKE,EIF6,PDHB,USP19,WDR20
Protein Size: 390 -
Molecular Weight
- 40 kDa
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Gene ID
- 5160
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NCBI Accession
- NM_000284, NP_000275
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UniProt
- P08559
Target
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