Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) Peptide
UBE2L3
Reactivity: Human
Host: Synthetic
BP, IHC, WB
Catalog No. ABIN985830
Quick Overview for Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) Peptide (ABIN985830)
Target
UBE2L3
(Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Immunohistochemistry (IHC), Western Blotting (WB)
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-UBE2L3 antibody (Catalog #: ARP43025_T100). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
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Background
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.
Alias Symbols: E2-F1, L-UBC, UBCH7, UbcM4
Protein Interaction Partner: UBE3A,ARIH1,ARIH2,BRCA1,CBL,CCNB1IP1,CUL9,NEDD4,NEDD4L,PARK2,PTEN,RNF144A,RNF144B,RNF19A,RNF216,SMAD4,UBE3A,UBOX5,ARIH1,ARIH1,ARIH2,ARIH2,AURKA,BRCA1,CBL,CBL,CUL9,EIF4E2,ITCH,NEDD4,POLR2A,PTEN,RNF19A,RNF216,RSP5,SMURF2,UBASH3A,UBE3A,UBOX5,Ubox5,WWP2
Protein Size: 668 -
Molecular Weight
- 75 kDa
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Gene ID
- 7332
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NCBI Accession
- NM_004739, NP_003338
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UniProt
- P68036
Target
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