PIP1,1/PIP1,2/PIP1,3 antibody
Quick Overview for PIP1,1/PIP1,2/PIP1,3 antibody (ABIN349671)
Target
Reactivity
Host
Clonality
Application
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Purpose
- Anti-PIP (PIP1,1, PIP1,2, PIP1,3, PIP1,4, PIP1,5) | Aquaporins
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Cross-Reactivity (Details)
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Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Setaria viridis, Thellungiella salsuginea
Not reactive in: Fragaria sp., Spinacia oleracea -
Predicted Reactivity
- Brassica sp.,Glycine max,Hordeum vulgare,Juglans regia,Medicago truncatula,Nicotiana tabacum,Oryza sativa,Populus tremula,Triticum aestivum,Vicia faba
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana: PIP1,1 UniProt: P61837
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Application Notes
- 1: 150 (IF), 1 : 1000 (WB)
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Comment
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Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20°C to -80°C.Manufactured by Operon Biotechnologies.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- PIP1,1/PIP1,2/PIP1,3 (Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3))
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Alternative Name
- PIP1 (PIP1,1, PIP1,2, PIP1,3) aquaporins
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Background
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Background: PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
Additional information: Antibodies will detect target protein in a few μg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one μg load per well should be sufficient
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Molecular Weight
- 30.68 | 28 kDa
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Gene ID
- 825316
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UniProt
- Q06611, Q08733, Q39196
Target
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