PIP1,1/PIP1,2/PIP1,3 antibody
This anti-PIP1,1/PIP1,2/PIP1,3 antibody is a Rabbit Polyclonal antibody detecting PIP1,1/PIP1,2/PIP1,3 in ELISA and WB. Suitable for Arabidopsis thaliana.
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Catalog No. ABIN349671
Quick Overview for PIP1,1/PIP1,2/PIP1,3 antibody (ABIN349671)
Target
PIP1,1/PIP1,2/PIP1,3
(Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 30.68 / 28 kDa
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Purification
- serum
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Immunogen
- synthetic peptideconserved in Arabidopsis thaliana: PIP1,1 P61837,At3g61430 PIP1,2 Q06611,At3g45960 PIP1,3 Q08733,At3g01620 , PIP1,4 Q39196, At3g00430, PIP1,5 Q8LAA6 At3g23400
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Application Notes
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Recommended Dilution: 1 : 8000 (ELISA), 1 : 1000 with standard ECL (WB).
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. -
Comment
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Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20°C to -80°C.Manufactured by Operon Biotechnologies.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 0.1 % sodium azide is added as preservative
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Storage
- -20 °C
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- PIP1,1/PIP1,2/PIP1,3 (Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3))
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Alternative Name
- PIP1 (PIP1,1, PIP1,2, PIP1,3) aquaporins
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Background
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AGI Code: At3g61430
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B, -
Molecular Weight
- expected: 30.68 kDa, apparent: 28 kDa
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UniProt
- Q39196, Q06611, Q08733
Target
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